Knockdown of EB3 by shRNA reduces the number of dendritic spines. a, Representative images of dendritic processes and spines in a control neuron expressing GFP and a neuron coexpressing shRNA-EB3 and GFP. Scale bar, 20 μm. b, Quantification of the spine numbers for the control and shRNA-EB3 expressing neurons. Asterisk indicates a statistical significance (p < 0.05, Student's t test). c, Measurements of the spine size (width and length).