Figure 5.

The Dcr-1-Ago1 complex is less abundant in the absence of dFMR1. A, Control mouse IgG or Ago1 antibody were used to immunoprecipitate Ago1-containing complexes from lysates of dissected wild-type (WT) or dfmr1 mutant larval brains. The presence or absence of Dcr-1 or dFMR1 was detected by Western blot. This experiment was repeated three times. B, Quantification of the abundance of Dicer-1 in Ago1 immunoprecipitates based on three independent experiments. The values are mean ± SEM. p < 0.01 versus wild type. C, The protein band recognized by the Dcr-1 antibody obtained from Abcam is absent in the dcr-1 mutants. D, An Ago1 antibody (Okamura et al., 2004) identifies Ago1 isoforms that are absent in ago1 mutants. Note that the expression levels of Dcr-1 and Ago1 are the same in dfmr1 mutants and wild-type larvae. IP, Immunoprecipation.