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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Cell Death Differ. 2008 Oct 10;16(1):175–183. doi: 10.1038/cdd.2008.143

Figure 5. Autophagy regulates selective release of HMGB1 from DT-EGF-treated cells.

Figure 5

Panel A, U87MG cells transfected with GFP-HMGB1 and control (non-specific) or Atg12 siRNAs were monitored by time-lapse microscopy after treatment with DT-EGF. DT-EGF causes release of HMGB1 in control cells, Atg12 knockdown causes the retention of HMGB1 in fragmented apoptotic nuclei. Panel B, western blot analysis of cell extract and media for HMGB1 protein after DT-EGF treatment for 48 hours. HMGB1 is not released into the media in DT-EGF-treated cells when autophagy regulators are knocked down. Control shows cells treated for 3.5 hours with 10mM H2O2.