Skip to main content
. Author manuscript; available in PMC: 2009 Jun 11.
Published in final edited form as: Nature. 2008 Nov 9;456(7223):809–813. doi: 10.1038/nature07424

Figure 3. An Inducible VEGF-R2/PDGF-Rβ Complex Forms in VSMC.

Figure 3

a, Immunoblot analysis of anti-PDGF-Rβ immunoprecipitated lysates from serum-starved VSMCs subjected to siRNA-mediated VEGF receptor knock-down. b, Upper: Immunoblot analysis of anti-PDGF-Rβ immunoprecipitated lysates from serum-starved NHDFs (VEGF-R2 null) infected with adenovirus. Immunoblots represent at least three similar experiments. Lower left: Representative proliferation (left) and migration (right) experiments with VEGF-R2 reconstituted NHDFs; *P<0.001, P<0.02, results from one-way ANOVA, n>3 separate experiments. c, Representative reciprocal co immunoprecipitation followed by immunoblot analysis with lysates derived from serum-starved VSMCs pre-treated with either control or VEGF-R2 inhibitor or d, serum-starved NHDFs transduced with truncated VEGF-R2 adenovirus or e, NHDFs transduced with VEGF-R2 catalytic site (Y1052F and Y1057F) and effector site mutants (Y1174F and Y1212F). IB, immunoblot; IP, immunoprecipitation; WCL, whole cell lysate; c-t, C-terminus. Immunoblots are representative of at least three similar experiments; mutant receptors are characterized in Supplementary Fig. 8.