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. 2008 Dec 22;205(13):3173–3185. doi: 10.1084/jem.20081427

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© 2008 Croquelois et al.

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Figure 6. — Notch1 activation in proliferating undifferentiated cardiac-committed cells in the adult myocardium. (A) Confocal microscopy of normal heart tissue sections showing activation of Notch1 (N1IC) in GATA4-positive α-actinin–negative cells. Nuclei are stained with DAPI. (B) Staining of cardiac tissue sections, as in A, showing N1IC staining in Nkx2.5-positive nuclei of undifferentiated α-actinin–negative cells. (C) Activation of Notch1 (N1IC) in proliferating Ki67-positive α-actinin–negative cells. (D) Analysis of BrdU incorporation in hearts of clipped (1K1C) and sham-operated (sham) WT mice and mice lacking Notch1 in the heart (N1^del). Tissue sections of hearts from mice administered with BrdU for 96 h in drinking water were stained with anti-BrdU antibody. The BrdU⁺ nuclei are red. (E) Quantitation of BrdU incorporation represented as percentage of BrdU⁺ nuclei. * and † denote significance at P < 0.01 within groups and between groups, respectively. Error bars show the mean ± SD. Bars: (A, B, and C) 10 μm; (D) 100 μm.