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. 2008 Dec 22;205(13):2985–2994. doi: 10.1084/jem.20082053

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© 2008 Panigrahi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — RS rearrangement levels are highest in late pre–B cells. (a) Vκ-RS rearrangement levels quantified in bone marrow subsets from C57BL/6 mice (n = 5). (b) Vκ-RS rearrangement levels assayed in less mature (BP-1⁺; gray bar) and more mature (BP-1⁻; black bar) Fr. D B cells of C57BL/6 mice (n = 3). All PCR reactions were performed in duplicate. Data are depicted as fold difference (+SEM; **, P ≤ 0.01) relative to the mean RS level in C57BL/6 splenic B220⁺ IgM⁺ κ⁺ B cells (dashed lines). Bone marrow B cell fractions were obtained by flow cytometry, as shown in Fig. S1 (available at http://www.jem.org/cgi/content/full/jem.20082053/DC1).

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