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. 2008 Dec 30;3(12):e4077. doi: 10.1371/journal.pone.0004077

Figure 5. SERPINA3K does not chelate free calcium.

Figure 5

Free [Ca2+] was measured by the fluorescence of the Fluo-4 (pentapotassium salt). (A) A Ca2+/Fluo-4 binding curve was plotted with different concentrations of CaCl2 and 10 µM Fluo-4. (B) SERPINA3K (10 µM) was incubated with 10 µM CaCl2 or a cell lysate in the presence of 10 µM Fluo-4. EGTA (10 µM) and BSA (10 µM) were used as positive and negative controls, respectively. Free [Ca2+] was measured (mean±SEM, n = 3). * P<0.01, versus control cells.