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. 2008 Dec 31;3(12):e4090. doi: 10.1371/journal.pone.0004090

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Pfister et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Effect of sirtuin expression on HT-22 cell survival. Sirtuin expression levels were enhanced in HT-22 neuroblastoma cells via transfection with mammalian expression plasmids. HT-22 cells were treated 24 hours following transfection with normal growth medium (untreated), 1 mM HCA or Serum-free (SF) medium for 24 hours. The viability of transfected cells was determined using a TUNEL assay. The mean of 5 experiments were taken (* indicates p<.05 compared with Flag untreated sample; # indicates p<.05 compared with Flag HCA sample; ˆ indicates p<.05 compared with Flag SF sample). (B) Subcellular localization of exogenous SIRT1, SIRT2, SIRT3, SIRT5 and SIRT6 in HT-22 cells. HT-22 cells were transfected with SIRT-Flag constructs and immunocytochemistry performed using a Flag antibody. DAPI staining was used to visualize nuclei/chromatin. SIRT5 shows colocalization with the mitochondrial protein Cox4.