Figure 4.
(a) Schematic design of PDMS embryo culture device. (b) Enlarged culture area. It consists of three layers; Upper channel (200μm height) for introducing embryo, middle channel for Braille system (30μm height) and PDMS-parylene-PDMS hybrid membrane with 80μm deep well to keep the introduced embryos in the culture chamber i.e. “Snake head” (300μm wide × 200μm depth).