Figure 4. Mucin O-glycan utilization PULs.
Four loci induced during growth in the presence of mucin O-glycans, and a fifth in vivo-specific system (BT1617-22). Note the presence of an extra N-terminal trans-envelope signaling domain on one of the susC homologs associated with each system (Koebnik, 2005). The anti-σ factor associated with the BT4240-50 locus was assembled in the VPI-5482 genome as two divergent genes (BT4248-49) but undergoes a DNA inversion that positions BT4249 upstream of BT4248, resulting in an intact anti-σ factor gene (see Fig. S12 for details). Gray boxes indicate genes for which elevated basal transcription was observed (see Supplemental Data). Delta (Δ) symbols below ECF-σ genes indicate that they were targeted for in-frame deletions for in vivo competition experiments.