Fig. 2.

JNK protein levels do not change after axotomy. Protein extracts prepared from injured and contralateral uninjured DRGs were immunoblotted for JNK at progressive times after proximal (•) or distal (▪) axotomy. The intensity of the JNK signal was measured by densitometric analysis of ECL autoradiographs, and a ratio between injured and contralateral DRGs was determined. Each data point is representative of the injured/contralateral JNK ratio in three different animals. Error bars represent SEM. There was no statistically significant difference in JNK levels between injured and contralateral, uninjured DRGs at any time, for either surgical procedure.