TABLE 2.
Differential effect of NGF and BDNF on spike waveform and GABA conductance in axotomized cutaneous afferent DRG neurons
| Control | Ringer Solution | NGF, 0.25 μg/μl | BDNF, 0.5 μg/μl | |
|---|---|---|---|---|
| Cell size, μm | 43 ± 0.6 (58) | 45 ± 1.05 (24) | 47 ± 0.64* (38) | 42 ± 0.84§ (20) |
| Cell cap, pF | 88 ± 3.50 (58) | 86 ± 4.23 (24) | 117 ± 4.92*† (38) | 79 ± 5.35 (20) |
| GGABA, nS | 287 ± 27 (58) | 605 ± 57* (24) | 618 ± 64* (38) | 365 ± 44† (19) |
| GGABA/cap, nS/pF | 3.53 ± 0.36 (58) | 7.45 ± 0.75* (24) | 5.65 ± 0.67* (38) | 5.17 ± 0.66‡§ (19) |
| RI, MΩ | 92 ± 6.9 (58) | 115 ± 22.6 (24) | 97 ± 9.6 (38) | 107 ± 21.1 (20) |
| RP, mV | −53 ± 0.9 (58) | − 58 ± 0.9* (24) | − 57 ± 0.6* (38) | −57 ± 0.8* (20) |
| AP half-width, ms | 1.09 ± 0.11 (58) | 0.76 ± 0.75* (22) | 1.07 ± 0.07† (38) | 0.97 ± 0.14 (20) |
| AP % inflected | 47 (27/58) | 18 (4/22) | 61 (23/38) | 25 (5/20) |
Values are means ± SE, with number of neurons in parentheses. GABA, γ-aminobutyric acid; DRG, dorsal root ganglion; AP, action potential. Parameters were compared in uninjured control neurons and axotomized neurons from animals in which the transected sciatic nerve was perfused with vehicle solution (Ringer solution), nerve growth factor NGF, 0.25 μg/μl), or brain-derived neurotrophic factor (BDNF, 0.5 μg/μl).
*
P < 0.01,
‡
P < 0.05 as compared with uninjured cutaneous afferents.
†
P < 0.01,
§
P < 0.05 as compared with axotomized, Ringer-solution-treated cutaneous afferents.