Fig. 1.

Multiple migratory pathways for neuroblasts are revealed in the postnatal brain of EGFP/5-HT₃ transgenic mice. (A) Overview of EGFP expression in a sagittal section of a P20 transgenic animal. (B) EGFP immunocytochemistry in sagittal sections from P10 transgenic animals showing the RMS, 2 novel pathways termed VMP and DMP, and cells detached from the RMS (Bottom Right). (C) EGFP immunohistochemistry showing the EMP on horizontal sections from P10 transgenic mice. (D–E″) Colocalization of EGFP (green) and DCX (red) in cells in the DMP (D–D″) and EMP (E–E″) in sagittal and horizontal sections, respectively, from P10 transgenic animals. (F and G) Colocalization of EGFP (green) and DCX (red) in the RMS, in cells detached from the RMS in P9 sagittal sections (F), and in migratory cells that have exited the EMP in P16 horizontal sections (indicated by arrows in G). (B, C, F, and G) Plane of sections is indicated in the Inset. [Scale bars: (A) 200 μm, (B) 50 μm, (C) 200 μm, (D–E″) 20 μm, (F) 50 μm, (G) 75 μm.]