Fig. 3.

BrdU birthdating and differentiation of postnatally generated EGFP-positive interneurons. (A and B) Location of EGFP/BrdU double-positive cells 72 h after BrdU injection at P4 in the DMP (A) and at the posterior edge of the alveus of the hippocampus (B). (C and D′) Numerous EGFP/BrdU-positive cells with migratory appearance in layer VI of the frontal cortex, 10 days after BrdU injections at P4. (D and D′) EGFP/BrdU-positive cell with morphology indicative for somal translocation. Leading and lagging process of the cell are indicated by arrows in D′. (E–F″) Triple stainings showing colocalization of EGFP (green), DCX (blue), and BrdU (red) in the secondary visual cortex (E and E′), striatum (F′), and around the anterior commissure (F″) 10 days after BrdU injections at P4. Within the striatum (F), both single EGFP-positive cells and an impressive number of chains of migratory cells (arrows) can be detected. (C′, D′, E′, F′, and F″) Enlargements of the boxed areas in C, D, E, and F, respectively. Colocalization is indicated by arrows (B, C′, E′, F′, and F″). (G) Triple stainings illustrating the colocalization of EGFP (green), NeuN (blue), and BrdU (red) in layer VI of the primary somatosensory cortex, 28 days after BrdU injections at P4. (H–I′) Examples of triple-labeled EGFP (green), CR (blue), and BrdU (red) cells in striatum (H) and in layer IV of the frontal cortex (I and I′), 28 days after BrdU injections at P4 (H) or at P7 (I and I′). (I′) Enlargement of Inset in I. Cx, cortex; Hi, hippocampus; CPu, caudate putamen. [Scale bars: (A, C, D, E, F, I) 200 μm; (C′, I′) 50 μm; (B, D′, E′, F′, F″, G, H) 10 μm.]