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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: J Cell Physiol. 2009 Jan;218(1):205–214. doi: 10.1002/jcp.21590

Fig. 3. Inhibition of MEFs growth by NiCl2.

Fig. 3

1 × 103 of MEFs were seeded into each well of 96-well plates, cultured in 10% FBS DMEM overnight, and then exposed to NiCl2 or NiS for 72 h. The cells were photographed under microscopy (a), and then extracted by lysis buffer for growth measurement using CellTiter-Glo® Luminescent Cell Viability Assay kit with a luminometer (b). The results are expressed as inhibition of cell growth which was calculated as described in Material and Methods. The symbol (♣) indicates a significant different from NiCl2 group (p< 0.05).