Fig. 5. HIF-1α is not involved in cyclin D1 degradation induced by NiCl₂.

HIF-1α^+/+ MEFs were seeded into each well of 6-well plates, and cultured in 10% FBS DMEM overnight and exposed to various concentrations of nickel compounds as indicated for 12 hrs, and then subjected to Western blot assay (a). HIF-1α^−/− MEFs were identified using HIF-1α antibody (b). The effect of nickel compounds on cyclin D1 protein expression were compared between HIF-1α^+/+ MEFs and HIF-1α^−/− MEFs by Western blot assay. CDK4 and β-Actin were used as controls (c).