Table 2.
Number of nucleotide and amino acid substitutions and Ka/Ks ratio of Malaysian CAV isolates
| CAV isolates | *No. of Nucleotide Substitutions | *No. of amino acid Substitutions | non-synonymous substitution rate (KA) | Synonymous substitution rate (KS) | Omega value (KA/KS) | References |
| MF1A | 28 | 6 | 0.017 | 0.199 | 0.08 | This study |
| MF3C | 30 | 6 | 0.017 | 0.225 | 0.07 | This study |
| M3B5 | 27 | 6 | 0.017 | 0.191 | 0.09 | This study |
| NF4A | 27 | 7 | 0.020 | 0.179 | 0.11 | This study |
| P12B | 27 | 6 | 0.017 | 0.187 | 0.09 | This study |
| P24A | 18 | 6 | 0.017 | 0.191 | 0.09 | This study |
| PPW4 | 12 | 7 | 0.020 | 0.039 | 0.50 | This study |
| NF1D | 13 | 6 | 0.017 | 0.053 | 0.33 | This study |
| NF2C | 13 | 6 | 0.017 | 0.055 | 0.31 | This study |
| NF3A | 11 | 4 | 0.012 | 0.047 | 0.25 | This study |
| PYT4 | 11 | 5 | 0.015 | 0.042 | 0.35 | This study |
| M1B1 | 9 | 4 | 0.012 | 0.036 | 0.32 | This study |
| SMSC-1 | 26 | 5 | 0.017 | 0.187 | 0.09 | [13] |
| 3-1 | 8 | 4 | 0.011 | 0.060 | 0.19 | [13] |
| BL-5 | 4 | 3 | 0.009 | 0.006 | 1.50 | [12] |
* compare to the consensus nucleotide and amino acid sequences. Pair wise comparison with the consensus nucleotide and amino acid sequence indicated Ka/Ks ratio less than one for all the studied isolates which indicates the occurrence of negative selection.