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. 2007 Feb 5;363(1489):185–198. doi: 10.1098/rstb.2006.2020

Figure 3.

Figure 3

Characterization and differentiation of precursors isolated from the rodent olfactory epithelium. (a) Immunocytochemical comparison of spheres grown from embryonic rodent cortex, post-natal rodent olfactory epithelium and post-natal rodent skin. Note that olfactory epithelium-derived spheres have an immunocytochemical phenotype intermediate to cortical and skin-derived spheres. For example, while only very rare cells from SKP spheres were positive for the p75 neurotrophin receptor (p75NTR) and many cells from CNS cortical neurospheres were positive, a significant fraction of cells from the olfactory epithelium also expressed this receptor. (b) Cells differentiating for 1 day from olfactory epithelium-derived spheres co-express nestin and fibronectin, similar to SKPs. (c) After 2–3 weeks of differentiation, olfactory epithelium-derived spheres generate neurons that express βIII tubulin, MAP2 and NFM, smooth muscle cells that express SMA, and glial cells that express CNPase and GFAP. MAP2, microtubule-associated protein-2; NFM, medium neurofilaments; SMA, smooth muscle actin; CNPase, cyclic nucleotide phosphohydrolase; GFAP, glial fibrillary acidic protein.