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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Fungal Genet Biol. 2007 Sep 21;45(4):527–541. doi: 10.1016/j.fgb.2007.08.008

Fig. 4.

Fig. 4

Genetic and functional analysis of the HIS4 alleles in strain CAI-4. A. Diagram showing the possibilities of integration of a disruption cassette prepared from the 1001 allele in CAI-4 cells. Alleles are distinguished with either light (inactive) or dark (active) grey. The presence of three heterologies between the upstream region of the URA blaster and the same region of the active allele is shown. We have indicated three heterozygosities in 5′ region of the disrupted functional allele (*) and labeled that region with an intermediate grey because we have not investigated the bases present in each disruptant (they may belong to either the cassette or the allele, depending on the recombination point). B. PCR of cognate heterozygous clones and sequencing data (for details see the text). For the sake of simplicity only two SNPs are shown but the other supports the expected haplotype. All strains were grown in SC with/without histidine to distinguish between prototrophs (His+) and auxotrophs (His−) (bottom line). C. Behavior of several strains in this assay.