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. Author manuscript; available in PMC: 2008 Dec 19.
Published in final edited form as: Front Biosci. 2007 Jan 1;12:2646–2657. doi: 10.2741/2261

Figure 5.

Figure 5

Cropped regions of images taken of in situ calcein-loaded articular chondrocytes at increasing image depths (30μm intervals) into cartilage (see Materials and Methods). At each depth and with each wavelength, both laser power and detector sensitivity were optimised to produce an image with the best contrast possible. For TPE the same field of view was imaged using internal ‘de-scanned’ (black) and non-de-scanned detectors (red). OPE (488nm) was as good as or better than TPE images acquired using internal ‘de-scanned’ detectors. Improvements in imaging depth were produced with NDDs, especially at 920nm TPE. The scale bar shown in the top left hand image (488nm, 30μm depth) represented 10 μm.