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Characterization of the primary and amplified cDNA libraries of pigeon.
| Number of primary libraries | Ligation ratio of cDNA to vector | Titer of primary libraries (pfu/mL) | Recombination efficiency of primary libraries (%) | Titer of amplified libraries (pfu/mL) | Recombination efficiency of amplified libraries (%) |
|---|---|---|---|---|---|
| (1) | 1:0.5 | 0.39×106 | 92.31 | --- | --- |
| (2) | 1:1 | 1.54×106 | 95.47 | 1.89×108 | 92.11 |
| (3) | 1:1.5 | 1.80×106 | 97.82 | 2.32×109 | 96.46 |
