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. 2008 Aug 25;9(8):1504–1514. doi: 10.3390/ijms9081504

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© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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EWSR1 interacts with TFIP11. HEK293 cells were transfected with plasmid expressing EWSR1-Myc (lanes 1, 3, 5 and 7), or EWSR1-Myc and TFIP-FLAG (lanes 2, 4, 6 and 8). The input panels (lanes 1, 2, 5 and 6) show protein in the cell lysate prior to immunoprecipitation. Cell lysates were immunoprecipitated using anti-FLAG monoclonal antibody (lanes 3, 4, 7 and 8), and then immunoblotted with either the anti-FLAG (lanes 1–4) or anti-Myc antibody (lanes 5–8). The plasmid pcDNA was cotransfected as a blank control to ensure equal concentrations of plasmid DNA being used for each transfection. Immunoprecipitate (IP), Immunoblot (IB).