Fig. 4.

Operon structure and transcription start site determination for the inrR transcript. (A) Gene localization and direction of the 4-gene operon containing inrR. (B) RT-PCR products with primers targeting regions a-e indicated in a from total RNA isolated from P. knackmussii strain B13 grown with 3-chlorobenzoate in stationary phase (Table S2). (C) Sequence of the region upstream of ORF95213 and indication of the 5′-end of the transcript (as “+1”). Corresponding −10 and −35 regions and the ribosome binding site (RBS) are overligned. Note the extended −10 TG motif typical for σ^s promoters (39).