Table 1.

Proportion of cells expressing both P_inR-echerry and P_int-egfp or P_inR-egfp and P_int-echerry in cultures of P. knackmussii strain B13

Incubation time,* h	PinR-echerry, Pint-egfp				PinR-egfp, Pint-echerry
	Proportion echerry†	Proportion egfp†	Percentage of common per echerry‡	Percentage of common per egfp‡	Proportion echerry†	Proportion egfp†	Percentage of common per echerry‡	Percentage of common per egfp‡
24	0.22 (±0.11)	0.09 (±0.06)	33	75	0.09 (±0.12)	0.48 (±0.28)	18	12
26	0.65 (±0.05)	0.40 (±0.26)	58	96	0.42 (±0.16)	1.1 (±0.08)	68	26
28	1.3 (±0.19)	1.1 (±0.09)	83	100	0.7 (±0.3)	1.8 (±0.2)	70	28
30	1.4 (±0.06)	1.1 (±0.07)	70	96	2.2 (±0.4)	2.9 (±0.6)	58	48
32	1.8 (±0.01)	1.4 (±0.09)	80	98	—	—	—	—
34	1.9 (±0.08)	1.7 (±0.01)	88	98	—	—	—	—
36	2.2 (±0.02)	2.0 (±0.06)	89	97	2.5 (±1.2)	3.8 (±0.8)	76	41
48	3.1 (±0.30)	3.3 (±0.36)	97	92	3.9 (±0.5)	3.9 (±0.3)	81	80
72	2.7 (±0.10)	2.9 (±0.41)	96	90	—	—	—	—
96	2.4 (±0.85)	2.5 (±0.94)	100	93	—	—	—	—

*P. knackmussii B13 (P_inR-echerry, P_int-egfp) or (P_inR-egfp, P_int-echerry) cells cultured in batch on minimal medium with 5 mM 3-chlorobenzoate as sole carbon and energy substrate. Incubation time denotes time of sampling since inoculation start; 24 h corresponds to early stationary phase. Cell samples immediately imaged under epifluorescence microscopy at two separate wavelengths for egfp and echerry.

^†Proportion of cells with fluorescence in echerry or egfp filter above background (Fig. S4 B-D) compared to total number of cells in sample (n > 1'500 cells). Values are average from triplicate samples of two independent P. knackmussii B13 (P_inR-echerry, P_int-egfp) or three (P_inR-egfp, P_int-echerry) clones (i.e., having different mini-Tn insertion positions).

^‡Percentage of cells having both echerry and egfp intensities above background per number of those having echerry or egfp.