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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Ann N Y Acad Sci. 2008 Dec;1147:335–347. doi: 10.1196/annals.1427.024

Fig. 3. SIRT1-mediated FoxO3a deacetylation is involved in FoxO3a modulation of Aβ generation.

Fig. 3

(A) Acetylation of FoxO3a decreases in the brain of CR treated Tg2576 transgenic mice. Endogenous FoxO3a was immunoprecipitated by anit-FKHRL-1 antibody followed by western blot using acetyl- lysine antibody. Total levels of FoxO3a were detected by western blot with anit-FKHRL-1 antibody. (B) SIRT1 deacetylates FoxO3a in cultured Tg2576 neurons. Tg2576 neurons were infected with SIRT1 adenovirus (MOI=10) for 48h. The acetylation of immunoprecipitated FoxO3a and total levels of FoxO3a were analyzed by western blot as described in (A). (C) Tg2576 neurons were infected with adeno-GFP or CA FoxO3a virus in combination with Ad-lacZ or Ad-WT SIRT1 viral infection. The resulting conditioned medium and cell lysates 48 hr post-transfection/infection were assessed for Aβ1-40 and Aβ1-42 concentrations. (C) Inset. Western blot analysis of SIRT1 and FoxO3a expressions. In (A) values represent means ± SEM, n = 4–5 per group. In (B) and (C), values represent means ± SEM of determinations made in three separate culture preparations; n=3 per culture preparation. In (A-C), *p<0.05 versus control group (2-tailed Student’s t test).