Figure 1. cPLA₂-α is present and levels are higher in androgen insensitive prostate cancer cell lines.

(A) Quantitative real time RT-PCR from the cell lines (Mean±S.D.) demonstrates cPLA₂-α expression in PC3 > DU145 > LNCaP (*p<0.05). (B) Staining on immunoblot of cell lines for total and p-cPLA₂-α was quantitated by densitometric analysis (Mean±SD) of protein expression on 3 separate immunoblots (*p<0.05 compared to LNCaP). (C) Protein levels of total and phosphor-cPLA₂-α on immunoblot. Staining on immunoblot was specific for the target protein as bands were absent when the primary antibody was preincubated with the specific blocking peptide.(D) Immunocytochemistry of control HeLa cells show strong staining for p-cPLA₂-α which is abolished with preincubation with blocking peptide. LNCaP and PC3 also show strong staining for p-cPLA₂-α. There is little staining with isotype control or method control in either cells line (PC3 shown). All photographs taken at X40, inserts magnified a further X4.