Figure 5. c-PLA₂-α inhibition impedes the growth of PC3 xenografts.

(A) PC3 cells were inoculated into the flanks of nude mice. When established xenograft tumors had reached 200m³ in size, the mice were randomized to control (vehicle) or Wyeth-1 treatment, given intraperitoneally daily at a dose of 10 mg/kg (8 mice/group). Statistically significant inhibition of tumor growth was achieved in the Wyeth-1 treated mice compared to the control animals (Mean±SEM, *p <0.05). (B) At the time of sacrifice, tumors were harvested and fixed in formaldehyde and paraffin embedded. Control tumors demonstrated significantly more cyclin D1 positive cells than did Wyeth-1 treated cells. (*p<0.05). (C) The tumors in control and Wyeth-1 treated mice stained for cyclin D1 and H&E. Tumors from Wyeth-1 treated mice, stained significantly less for cyclin D1 than control. H&E staining demonstrated very little necrosis in control tumors but striking necrosis in the Wyeth-1 treated tumors.