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. Author manuscript; available in PMC: 2008 Dec 19.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2008 Jun 5;28(8):1454–1461. doi: 10.1161/ATVBAHA.107.159392

Figure 3.

Figure 3

Pharmacological inhibition of S1P1 and S1P3, not S1P2, prevents S1P-induced SMC proliferation. Rat aortic SMCs were serum-starved for 72 hours and pretreated with vehicle (veh), VPC25239 (1.0 µmol/L, S1P1/S1P3 antagonist), or JTE013 (1.0 µmol/L, S1P2 antagonist) for 30 minutes followed by S1P treatment. Cell proliferation was assessed by changes in cell number. A, Dose response to S1P in the presence of veh, VPC25239, or JTE013 after 48 hours of stimulation with S1P. B, Proliferation response over 96 hours, media/reagents were changed after 48 hours. *S1P treatment > veh or S1P+VPC25239 (P<0.05). #S1P+JTE013 > veh, S1P, or S1P+VPC25239 (P<0.05).