Figure 8.

CR partly recovers the age-induced defective type I IFN signaling response in pDC. A-B, The pDCs harvested from CR aged mice exhibit reduced oxidative stress (grey line) at rest and during 18h of activation with CpG-A as compared to pDCs harvested from AL fed aged controls (black line). Young AL fed controls are shown (dashed line). Data represent at least four independent experiments with similar results (N=3/group). C-D, The pDCs harvested from CR aged mice displayed augmented IFN-α responses during in vitro activation with 18h CpG-A or in vivo infection with HSV-2 as compared to pDCs from AL-fed aged and young controls. Data represent at least two independent experiments with similar results (N=3/group). E, The pDCs from CR aged mice exhibit increased IRF-7 gene expression during either CpG-A or IFN-α stimulation as compared to pDCs from AL-fed aged mice. Gene expression from pDCs harvested from young AL-fed controls is shown. Data represent at least three independent experiments with similar results (N=3/group). F, The pDCs from CR aged mice exhibit increased IRF-7 activity, expressed as absorbance per μg nuclear extract, during CpG-A activation compared to pDCs from age-matched AL-fed mice. The pDCs from young AL-fed mice are shown. G, The pDCs from CR aged mice exhibit increased PI3-kinase upregulation during either CpG-A or IFN-α stimulation as compared to pDCs from ALfed aged mice. Gene expression from pDCs harvested from young AL-fed controls is shown. Data represents at least three independent experiments with similar results (N=3/group). H, The pDCs from CR aged mice exhibit increased phosphorylation of AKT during CpG-A activation compared to pDCs from age-matched AL-fed mice. The pDCs from young AL-fed mice are shown.