Figure 1.
p12 antisense inhibits TGF-β-induced EMT on HaCaT cells. A, Phase-contrast images of p12 antisense transfectants of HaCaT cells. Zn-inducible p12 antisense transfectants (ip12(−)HaCaT) and vector control transfectants (pMTCB6+-HaCaT) were cultured in DMEM + 5% FBS in the presence of 25 μM ZnSO4, and treated with or without a mixture of TGF-β (10 ng/ml) and EGF (30 ng/ml) for 48 h. B, Western blotting analysis of the E-cadherin and p12 protein levels in vector and p12 antisense transfectants with β-actin as a loading control.