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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Cancer Res. 2008 Dec 15;68(24):10377–10386. doi: 10.1158/0008-5472.CAN-08-1444

Figure 4.

Figure 4

p12-induced EMT enhances cell motility and local tumor invasion. A, Migration of vector and p12 transfectants from wounded edges in an in vitro cell migration assay. B, Average distances of cell migration in 24 h. A total of 100 cells were measured under a phase contrast microscope. C, H&E staining showed local invasion of the primary tumors derived from p12 transfectants (EMT) but not from vector transfectants (non-EMT). Tumor cells were indicated by stars. Double IF of tumor cells (indicated by stars) and blood vessels (indicated by arrows) showed intravasation of tumor cells only in the tumor tissues derived from p12 transfectants. Macro photos of the lungs and H&E examinations did not detect metastasis in the lung in all the animals in both groups (n=12). D, PCR analysis of GFP and DsRed genomic DNA collected from blood samples of the tumor-bearing mice.