Ability of non-EMT, EMT, and MET cells to establish lung metastasis from intravenous inoculation. A, All the animals (n=6) developed lung metastases from intravenous inoculation of vector transfectants (non-EMT cells) as shown by macro photos and H&E staining at both low and high magnifications. None of the animals developed lung metastasis from intravenous inoculation of p12 transfectants (EMT cells) and E-cadherin re-expressed p12 transfectants (MET cells). B, Western blotting analysis of E-cadherin confirmed E-cadherin re-expression. C, E-cadherin re-expression reverted the fibroblastoid structures of p12 transfectants to epithelial structures, suggesting an MET process.