Table 3.
Primers used in real-time RT-PCR assay
| Probe ID | Accession number | Description | Forward primers | Reverse primers |
| Mtr.47631.1.S1_s_at | 1645.m00036 | Transposase | 5'-CGTTACCCTGTTTTGGCAACA-3' | 5'-GCTCTCCGAAGCAACTGATGA-3' |
| Mtr.15107.1.S1_at | 775.m00015 | RNA-directed DNA polymerase | 5'-CCAATTGATAAAAGTGGTGCAAAT-3' | 5'-TGACTCCCTTTGATCCTGTAGCT-3' |
| Mtr.45925.1.S1_s_at | 740.m00009 | GH | 5'-TGACTGAAACGTTAACTGGAGTAAGG-3' | 5'-TTTCAAAGGTAATCCTCCTAGCAAA-3' |
| Mtr.43735.1.S1_at | TC95950 | MtRR1 | 5'-TGAAACGGAGCTGGTGATGA-3' | 5'-CAATCTCACAGGTTTCAGCAGAA-3' |
| Mtr.47174.1.S1_at | 1693.m00050 | AGL20 | 5'-AGAACAGCAGTTGGAAAAGAGTGTT-3' | 5'-TTTTAGTTGGTCAATTTGATGCTTGT-3' |
| Mtr.41073.1.S1_at | TC108662 | FPF1 | 5'-TTGGTCGAGAATCCTCAAGCA-3' | 5'-TGTGGGCAAGTGAACCAACA-3' |
| Mtr.8427.1.S1_at | TC100141 | LipOx | 5'-AGCCTTGGTGGCCTGAGAT-3' | 5'-CGGATGCAAGCCATATGATTATT-3' |
| Mtr.10439.1.S1_at | TC106784 | EIN3 | 5'-CGATTAAAGGAGCAAGTCAAACC-3' | 5'-TTGCCTGTTCCTGGGATTG-3' |
| Mtr.8585.1.S1_at | TC100726 | MtN3 | 5'-TGATGTTGTGAAGATTGGAACAGA-3' | 5'-TGGATCCCATGTTAAAATCAGACTT-3' |
| Mtr.18380.1.S1_at | 949.m00022 | Fasciclin | 5'-CCTAGTGATTCCACCCCTGACA-3' | 5'-GCCTTCGCCTTTCTCAGGAT-3' |
| Not on the array | TC100142 | Ubiqutin10 | 5'-GAACTTGTTGCATGGGTCTTGA-3' | 5'-CATTAAGTTTGACAAAGAGAAAGAGACAGA-3' |
Accession numbers starting with TC are from TIGR gene Index (MtGI) otherwise from the annotation by the International Medicago Genome Annotation Group (IMGAG). GH, Glycoside hydrolase; RR, response regulator; AGL20, agamous-like 20; FPF, flower promoting factor; LipOx, lipoxygenase; EIN, ethylene-insensitive; MtN3, Medicago truncatula nodulin3.