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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Stem Cells. 2008 Sep 11;26(12):3257–3266. doi: 10.1634/stemcells.2008-0258

Figure 2.

Figure 2

Composite FB element can effectively block the activity of the CMV enhancer in an in vitro enhancer blocking assay. (A) Schematic diagram showing the reporter plasmids used in the flow-cytometry based enhancer blocking assay. Abbreviations: DSR4, DsRed.T4; CMV-E, human CMV immediate early region enhancer; FB, the FII enhancer blocking component of the chicken β-globin 5′ HS4 insulator and the BEAD-A homologous region from the human T-cell receptor α/δ BEAD-I insulator; TK, herpes simplex virus thymidine kinase promoter; PA, SV40 late poly(A) signal. Other abbreviations as described in the text. (B) The constructs shown on the left were used in either transient transfections of 293T cells (upper) or stable transfections of K562 cells (lower). Relative GFP expression (normalized to DsRed.T4 expression) and the percentage of enhancer blocking for each construct are shown for triplicate determinations.