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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 May 23;49(10):4384–4391. doi: 10.1167/iovs.08-1688

FIGURE 9.

FIGURE 9

Electron micrographs of unwounded, central stromas stained with cuprolinic blue. Tissue was stained with cuprolinic blue before it was processed for electron microscopy. (A) Micrographs of middle stroma. Closed arrows: PGs associated with collagen fibrils. Open arrows: elongated filaments running along fibrils. Arrowheads: elongated filaments seen in cross-section. Scale bar, 250 nm. (B) PU/collagen fibril ratio in WT and P2X7 −/− stromas. The length of collagen fibrils was measured, and the number of PG units along that length was counted. PG per micrometer is graphed ± SEM. At last 22 measurements were performed for each group. *P < 0.001; t-test.