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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Jan 17;1777(3):317–326. doi: 10.1016/j.bbabio.2008.01.005

TABLE 1.

Characterization of mutants. The cytochrome bc1 complexes were in 50 mM Tris-Cl, pH 8.0, containing 200 mM NaCl, 200 mM histidine, 0.5% octyl glucoside and 10% glycerol.

Strains Ps a Activity b
Chromatoxphore Purified complex
Wild type + + + 2.3 (100%) 2.5 (100%)
S141C(ISP) + + + 2.3 (100%) 2.5 (100%)
G180C(cyt c1) + + + 1.8 (78%) 2.0 (80%)
S141C(ISP)/G180C(cyt c1) + + 0.6 (26%) 0.2 (8%)*
H111C(cyt c1) + 0.2 (9%) 0.6 (24%)
S141C(ISP)/H111C(cyt c1) + 0.2 (9%) 0.6 (24%)
G112C(cyt c1) + 0.8 (35%) 0.9 (36%)
S141C(ISP)/G112C(cyt c1) + 0.8 (35%) 0.9 (36%)
M114C(cyt c1) + + + 2.3 (100%) 2.5 (100%)
S141C(ISP)/M114(cyt c1) + + + 2.3 (100%) 2.5 (100%)
a

Ps=photosynthetic growth.

b

The enzymatic activity is expressed as μmol of cytochrome c reduced/min/nmol cytochrome b at room temperature.

*

If purification is carried out in the presence of β-ME, this value will be 2.0.