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. 2008 Nov 12;416(Pt 2):263–270. doi: 10.1042/BJ20081417

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© 2008 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Three typical HPLC profiles from three assay tubes are illustrated, with the radioactivity in the InsP₇ region (indicated by the peak in [³H]InsP₇ elution) shown. Left-hand axis (♦), [³H]; right-hand axis (■), [³²P]. Note the differing scales on the [³²P] axes [dpm (d.p.m.)]. (A) Blank incubation (control), (B) extract of 100 μl of human plasma, and (C) extract of 100 μl of human plasma with 2.5 pmol (25 nM final concentration) InsP₆ added before extraction.