Figure 2.

Only synapsin IIa rescues synaptic depression in TKO neurons. A, EPSCs recorded in an untransfected TKO neuron and in a TKO neuron transfected with synapsin IIa. Depression was elicited by 10 Hz trains of stimuli and the traces show every 15th EPSC evoked by the train. B, Mean EPSC amplitudes, normalized to the first response, with every 30th response shown. Lines represent single-exponential functions fitted beginning at the 30th response; note the semilogarithmic scale. C, Mean time constants of synaptic depression of TKO (n = 28) and TWT (n = 28) neurons, and of TKO neurons expressing synapsins Ia (n = 11), Ib (n = 12), IIa (n = 16), IIb (n = 17), and IIIa (n = 18). Only synapsin IIa significantly (*p < 0.05) slows synaptic depression compared with the TKO background.