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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: Dev Biol. 2008 Apr 8;318(2):323–334. doi: 10.1016/j.ydbio.2008.03.037

Figure 3.

Figure 3

Twist1+/− sutures are more responsive to FGF. (A) E16.5 calvarias stained with Alcian Blue and Alizarin Red. Note that wild type and Twist1+/− calvaria are phenotypically the same at this stage. The drawing in the middle shows the location of beads (red dots) that were placed on the osteogenic fronts (OF) or on the mid-sutures (MS). (B) BSA or FGF2-soaked beads were placed on the osteogenic fronts or mid-sutures of wild type and Twist1+/− E16.5 calvaria, and the explants were cultured for 7 days. FGF2 beads placed on the osteogenic fronts induced suture closure in both wild type and Twist1+/− explants, while only the Twist1+/− sutures closed when FGF2 beads were placed on the mid-sutures. (C) Table showing the results of beads placed either on osteogenic fronts or mid-suture of calvaria explants of wild type versus Twist1+/− mice. Suture closure was defined as when the two parietal bones came in contact.