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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Osteoarthritis Cartilage. 2008 Jun 18;16(12):1560–1571. doi: 10.1016/j.joca.2008.04.027

Figure 5. Reversal of IL-1β-induced phenotype by TGF-β1.

Figure 5

Chondrocytes from normal knee cartilage and preserved areas of OA knee cartilage were exposed to 0.1 ng/ml of IL-1β for 24 h. Control cells (C) received vehicle alone. After 24 h, media containing IL-1β was removed and fresh media containing 10 ng/ml of TGF-β1 was added for 48 h. RNA samples were analyzed by RT-PCR with indicated primers. GAPDH was used as reference for gel loading. Lanes denoted as (IL-1β) indicate that cells were treated with IL-1β for 24 h and were not treated with TGF-β1; lanes denoted as TGF-β1 (IL-1β) indicate cells received TGF-β1 after removal of IL-1β; ^ indicates that the bands were too low to quantify; ~ indicates no change.