FIGURE 2.

Glutathione depletion is stimulated by MK571 and prevented by high extracellular GSH. Intracellular GSH measurements by FACS were performed and represented as in Fig. 1B. Apoptosis was induced by 25 ng/ml FasL (4 h), in the presence or absence of 50 μm MK571 and high extracellular GSH medium (+GSH). High glutathione (+GSH) medium was made by substituting NaCl with 25 mm l-glutathione, maintaining the same osmolarity of the media. Plots are representative of at least four independent experiments.