FIGURE 11.
Wild type, but not GoLoco-insensitive, Gαil-YFP destabilizes metaphase chromosomes and spindle orientation. Time-lapse images of Hoechst 33342-stained chromosomes during metaphase alignment and segregation were recorded as described (32); supplemental movies are available. Representative consecutive fluorescence images taken from time-lapse sequences showing the motion of Hoechst-stained chromosomes in MDCK II cells expressing Gαi1(wild type)-YFP (upper panel) and Gαi1(N149I)-YFP (lower panel). The images were taken every 3 s as described (32). To show the movements of the metaphase chromosomal arrays, the position of the long axis of the chromosomal array along the metaphase plate in each image was marked by red (upper panel) or blue lines (lower panel), and positions of the axis in previous adjacent images are marked with white lines.