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Wild type Gαil, but not GoLoco-insensitive Gαil, causes membrane localization of endogenous GPSM2. MDCK II cells were transfected with KT3-epitope tagged Gαi1(wild type) (top panel) or Gαi1(N149I) (bottom panel). Twenty four hours later, cells were fixed and stained with anti-GPSM2 antibodies, anti-KT3 antibodies, and DNA was stained with 4′,6-diamidino-2-phenylindole. Images were obtained using confocal microscopy.
