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. 2008 Dec 26;283(52):36698–36710. doi: 10.1074/jbc.M804936200

FIGURE 9.

FIGURE 9.

GPSM3-YFP translocates to the plasma membrane after overexpression of transmembrane domain-tethered wild type, but not GoLoco-insensitive Gα subunits. Confocal images of HEK 293 cells transiently expressing GPSM3-YFP and either vector control (pcDNA; left panels), CFP-TM-GαoA/i1 (middle panels), or CFP-TM-GαoA/i1 N149I (right panels). CFP fluorescence micrograph is shown above the corresponding YFP fluorescence micrograph for the same cells. GPSM3-YFP fluorescence is distributed throughout the nucleus and cytoplasm in control cells and in cells expressing CFP-TM-GαoA/i1 with the GoLoco-insensitivity N149I mutation, whereas GPSM3-YFP fluorescence is enriched at the plasma membrane in cells expressing CFP-TM-GαoA/i1. Average profiles of fluorescence intensity derived from lines drawn normal to the plasma membrane are plotted below the micrograph panels. Peaks of CFP intensity document comparable expression of CFP-TM-GαoA/i1 (n = 10 cells) and CFP-TM-GαoA/i1 N149I (n = 10 cells). a.u., arbitrary units.