TABLE 1.

Ectopic expression of wild type, but not GoLoco-insensitive, Gα_il-YFP destabilizes spindle orientation

Time-lapse images of Hoechst 33342-stained chromosomes during metaphase of MDCK II cells expressing Gα_il(wild type)-YFP or Gα_il(N149I)-YFP were recorded as described (32). Consecutive fluorescence images were taken every 3 s during mitosis (see supplemental movies 1-4 for example). The angles of the long axis of the chromosomal arrays in each frame (see Fig. 11 for examples) were measured using Metamorph software. The absolute angle changes of at least 100 sets of adjacent frames were binned into three categories: <5°, between 5 and 10°, and >10°. Data are the mean (S.E.M.) values obtained from three independent time-lapse analyses for either wild type or N149I Gα_il-YFP-expressing cells.

Angle change during spindle rocking	Relative frequency of observation (%)
	Wild type Gαil-YFP	N149I Gαil-YFP
0-5°	45 (3)	94 (2)
5-10°	38 (4)	6 (2)
>10°	17 (2)	0