FIGURE 12.

NKA function following D_2L DAR transfection. HEK293T cells were transfected with 7.5 μg of either D_2L cDNA or empty pcDNA vector (UTs). Cells were washed 24 h later, and for cells involving pertussis toxin treatment (PTX), 125 ng/ml PTX was added to media 16 h prior to experimentation. 48 h post-transfection, cells were removed into assay buffer and ⁸⁶Rb⁺ uptake, in the presence or absence of dopamine (DA), was measured as described under “Experimental Procedures.” Data were normalized for individual experiments as a percentage of the E_max value for UT cells alone and are expressed as mean ± S.E. of five independent experiments, each conducted in duplicate. All statistical analyses were performed using one-way ANOVA with Bonferroni's post-test; ^*, p < 0.05; ^**, p < 0.01. UT raw E_max values ranged from 109 to 219 pmol/mg protein/min.