FIGURE 1.

Activity profiles of human BRCA2 promoter luciferase reporter constructs in MCF-7 cells. A, schematic diagram of pGL3Prom, Del-9, Del-10, and a series of Del-9 mutants. B, luciferase activity profiles of the BRCA2 promoter reporter constructs in MCF-7 cells. To control for transfection efficiency, cells were co-transfected with pRL-TK, and the activity associated with each construct was normalized relative to Renilla luciferase activity. The luciferase activity for each construct is shown relative to the wild-type pGL3Prom construct.