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Whole mount and section in situ hybridization for components of the Shh pathway in TβRIIfl/fl and TβRIIΔ/Δ E12.5 (A–F) and E15.5 (G–L) lungs. Abundance of mRNA for both Ptc and Gli-1 is increased in the mutant lungs (E, F, K, and L) compared with the control lungs (B, C, H, and I). Little if any change is detectable in the epithelial-localized Shh (A, D, G, and J). Scale bar, 2.2 mm (A, B, E, F, I, J); 100 μm (C, D, G, H, K, L).
