Co-immunoprecipitation of IMPDH1(546)-GFP and rhodopsin mRNA. HEK293
cells were co-transfected with constructs directing the expression of
IMPDH1(546)-GFP and rhodopsin mRNA. IMPDH1(546)-GFP was immunoprecipitated
with anti-GFP antibody. A, Western blot of the immunoprecipitate
showing efficient pulldown of IMPDH1(546)-GFP. HEK cells transfected with
constructs expressing only rhodopsin (-) and rhodopsin and IMPDH1(546)-GFP
(+); ft, flow-through. B, reverse transcription
(RT) followed by PCR demonstrates the presence of rhodopsin mRNA in
the immunoprecipitate. Rho, rhodopsin; ft, flow-through.