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. 2008 Dec 26;283(52):36354–36360. doi: 10.1074/jbc.M806143200

FIGURE 7.

FIGURE 7.

Co-immunoprecipitation of IMPDH1(546)-GFP and rhodopsin mRNA. HEK293 cells were co-transfected with constructs directing the expression of IMPDH1(546)-GFP and rhodopsin mRNA. IMPDH1(546)-GFP was immunoprecipitated with anti-GFP antibody. A, Western blot of the immunoprecipitate showing efficient pulldown of IMPDH1(546)-GFP. HEK cells transfected with constructs expressing only rhodopsin (-) and rhodopsin and IMPDH1(546)-GFP (+); ft, flow-through. B, reverse transcription (RT) followed by PCR demonstrates the presence of rhodopsin mRNA in the immunoprecipitate. Rho, rhodopsin; ft, flow-through.