TABLE 1.
125I-Aβ-(1-40) hydrolyzing activity of IgVs following metal affinity and anion exchange FPLC purification
Recombinant IgV preparations purified by one round of metal affinity chromatography on nickel-agarose (preparation MA1) were subjected either to a second round of metal affinity chromatography (MA2) or anion exchange chromatography (AEQ) on a Mono Q FPLC column. Aβ hydrolysis was assayed as in Fig. 2. Shown are specific activities expressed in cpm/h/μg of protein.
|
Catalyst
|
Specific activity, cpm/h/μg of protein IgV (mean ± S.D.)
|
||
|---|---|---|---|
| MA1 | MA2 | AEQ | |
| IgVL2-t 2E6 | 1318 ± 375 | 1220 ± 134 | 4495 ± 1035 |
| IgVL-t′ 5D3 | 2660 ± 114 | 2273 ± 308 | 61401 ± 5274 |